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e2 ubch5b ube2d2 hy p79449 mce  (MedChemExpress)


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    Structured Review

    MedChemExpress e2 ubch5b ube2d2 hy p79449 mce
    E2 Ubch5b Ube2d2 Hy P79449 Mce, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+ubch5b+ube2d2+hy+p79449+mce/UbcH5b%2FUBE2D2%2C+Human/10__21203_slash_rs__3__rs___7102103_slash_v1-277-22-25
    Average 93 stars, based on 1 article reviews
    e2 ubch5b ube2d2 hy p79449 mce - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    In Vitro:

    Article Title: ALDH2 Suppresses Glycolysis in Hepatocellular Carcinoma via TRIM21-Mediated GLUT1 Degradation
    Article Snippet: After 48 h, cells were lysed and recombinant proteins were puri ed using HA-tag or V5-tag a nity beads (P2185S, P2187S; Beyotime), following the manufacturer’s instructions. .. In vitro ubiquitination assay Ubiquitination reactions were performed in a 60 μL system containing 100 ng E1 (UBE1, 20433ES25, Yeasen), 500 ng E2 (UbcH5b/UBE2D2, HY-P79449, MCE), 500 ng puri ed E3 ligase (HA-TRIM21 or HATRIM21ΔR), 2 μg ubiquitin (20431ES08, Yeasen), 1 μg recombinant substrate protein (V5-GLUT1-His), and 1 μg GST or GST-ALDH2 as indicated. .. All reactions were carried out in ubiquitination buffer (Mg/ATP buffer, Yeasen) supplemented with 5 mM MgCl2 and 2 mM ATP.

    Ubiquitin Proteomics:

    Article Title: ALDH2 Suppresses Glycolysis in Hepatocellular Carcinoma via TRIM21-Mediated GLUT1 Degradation
    Article Snippet: After 48 h, cells were lysed and recombinant proteins were puri ed using HA-tag or V5-tag a nity beads (P2185S, P2187S; Beyotime), following the manufacturer’s instructions. .. In vitro ubiquitination assay Ubiquitination reactions were performed in a 60 μL system containing 100 ng E1 (UBE1, 20433ES25, Yeasen), 500 ng E2 (UbcH5b/UBE2D2, HY-P79449, MCE), 500 ng puri ed E3 ligase (HA-TRIM21 or HATRIM21ΔR), 2 μg ubiquitin (20431ES08, Yeasen), 1 μg recombinant substrate protein (V5-GLUT1-His), and 1 μg GST or GST-ALDH2 as indicated. .. All reactions were carried out in ubiquitination buffer (Mg/ATP buffer, Yeasen) supplemented with 5 mM MgCl2 and 2 mM ATP.

    Hemagglutination Assay:

    Article Title: ALDH2 Suppresses Glycolysis in Hepatocellular Carcinoma via TRIM21-Mediated GLUT1 Degradation
    Article Snippet: After 48 h, cells were lysed and recombinant proteins were puri ed using HA-tag or V5-tag a nity beads (P2185S, P2187S; Beyotime), following the manufacturer’s instructions. .. In vitro ubiquitination assay Ubiquitination reactions were performed in a 60 μL system containing 100 ng E1 (UBE1, 20433ES25, Yeasen), 500 ng E2 (UbcH5b/UBE2D2, HY-P79449, MCE), 500 ng puri ed E3 ligase (HA-TRIM21 or HATRIM21ΔR), 2 μg ubiquitin (20431ES08, Yeasen), 1 μg recombinant substrate protein (V5-GLUT1-His), and 1 μg GST or GST-ALDH2 as indicated. .. All reactions were carried out in ubiquitination buffer (Mg/ATP buffer, Yeasen) supplemented with 5 mM MgCl2 and 2 mM ATP.

    Recombinant:

    Article Title: ALDH2 Suppresses Glycolysis in Hepatocellular Carcinoma via TRIM21-Mediated GLUT1 Degradation
    Article Snippet: After 48 h, cells were lysed and recombinant proteins were puri ed using HA-tag or V5-tag a nity beads (P2185S, P2187S; Beyotime), following the manufacturer’s instructions. .. In vitro ubiquitination assay Ubiquitination reactions were performed in a 60 μL system containing 100 ng E1 (UBE1, 20433ES25, Yeasen), 500 ng E2 (UbcH5b/UBE2D2, HY-P79449, MCE), 500 ng puri ed E3 ligase (HA-TRIM21 or HATRIM21ΔR), 2 μg ubiquitin (20431ES08, Yeasen), 1 μg recombinant substrate protein (V5-GLUT1-His), and 1 μg GST or GST-ALDH2 as indicated. .. All reactions were carried out in ubiquitination buffer (Mg/ATP buffer, Yeasen) supplemented with 5 mM MgCl2 and 2 mM ATP.



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    93
    MedChemExpress e2 ubch5b ube2d2 hy p79449 mce
    E2 Ubch5b Ube2d2 Hy P79449 Mce, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+ubch5b+ube2d2+hy+p79449+mce/UbcH5b%2FUBE2D2%2C+Human/10__21203_slash_rs__3__rs___7102103_slash_v1-277-22-25
    Average 93 stars, based on 1 article reviews
    e2 ubch5b ube2d2 hy p79449 mce - by Bioz Stars, 2026-10
    93/100 stars
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